Adsorption of Ethidium Bromide with activated charcoal:
1. pre-packaged
filter units:
are made
by Schleicher and Schuell (S&S Extractor™)
- available
from Mandel Scientific for about CDN $20 each or Sigma Chemical
Company (CDN $27.10 each).
- able to
absorb at least 5 mg of EB in solution containing 0.5 µg/mL
(capacity of at least 10L according to instructions)
- reduces
EB to less than 0.8% of original concentration (spectroscopic
assay)
- after filtration,
the solution can be flushed down the drain
- the charcoal
filter is bagged, tagged and disposed of by EH&S
2. charcoal
absorption in bulk solution: (reference 1)
- dilute
EB solution to less than 10 µg/mL
- for a volume
of 100 mL (1 mg EB)
- add 300
mg of activated charcoal
- stir for
20h at room temperature
- recover
charcoal containing EB by filtration, flush filtrate down the
drain
- put filter
paper and charcoal containing EB into a plastic bag for disposal
by EH&S
- expect
EB concentration in filtrate to be less than 0.05 µg/mL
(<0.5% original concentration) if inintiall solution is less
than 10 µg/mL
- system
effective with EB in water or buffer solution (MOPS or TBE)
or saturating concentration of cesium chloride.· reference
suggests that adsorption may be complete in an hour, but for
safety, leave for 20h before filtering.
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