Genome 36(4):712-724

The Genetic and RFLP Characterization of the Left End of Linkage Group III in Caenorhabditis elegans

Dave Pilgrim

MRC Laboratory of Molecular Biology, hills Road, cambridge, U.K. CB2 2QH
and
Department of Genetics, University of Alberta, Edmonton, AB. T6G 2E9

A genetic approach was taken to identify new transposable element Tc1-dependent polymorphisms on the left end of linkage group II in the nematode Caenorhabditis elegans. The cloning of the genomic DNA surrounding the Tc1 allowed the selection of overlapping clones(from the collection being used to assemble the physical map of the C. elegans genome). A contig of approximately 600-800kbp in the region has been identified, the genetic map of the region has been refined, and 10 new RFLPs as well as at least four previously characterized genetic loci have been positioned onto the physical map, to the resolution of a few cosmids. This analysis demonstrated the ability to combine physical and genetic mapping for the rapid analysis of large genomic regions (0.5-1 Mbp) in genetically amenable eukaryotes.