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Movies on the life of Parelaphostrongylus odocoilei

 P odocoilei L1 collection  One procedure for recovering first stage larvae (L1s) from feces is the "beaker technique" developed by Sean Forrester and Murray Lankester. A screed sac containing fecal pellets is placed in a small beaker of water. Larvae fall to the bottom. After removing the screen and allowing the contents to settle, the supernatant is removed by vacuum aspiration, leaving a small amount of water and all of the larvae. This scene illustrates the vacuum aspiration step.  Windows media download
~ 0.4 mb
 Quicktime download
~ 1.4 mb
 P odocoilei L1s  A deer infected with P. odocoilei may shed many thousand L1s per gram of feces. Performing the beaker recovery technique on even a small number of pellets can result in recovery of large numbers of larvae. As shown in this scene, the larvae are quite active. It is this activity that moves them off pellets, either during the artificial situation of the "beaker technique", or in nature as L1s disperse from feces to contaminate the surrounding area. (Differential interference contrast microscopy.)  Windows media download
~ 0.4 mb
 Quicktime download
~ 1.4 mb
 P odoocoilei single L1  Nematodes move by alternate contractions of left and right body wall musculature, resulting in sinusoidal movement.  Windows media download
~0.4 mb
 Quicktime download
~ 2.4 mb