Agarose Gel Electrophoresis |
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Part A: Set up the gel apparatus. Notice that we test the depth of the wells with the spacer - the small side will fit under the teeth of the comb easily while the larger side does not. Part B: Obtain some molten agarose, swirl gently (avoid bubbles), making sure the aragose is warm to the touch but not too hot. Pour the molten agarose in apparatus, let cool. Part C: Remove comb and put gel into the running box. Pour buffer on top of the gel. Part D : Load samples carefully and start electrophoresis. Part E : Notice how the dye has been separated into three bands. In this experiment, the direction of travel was from right (the cathode) to left (the anode). |