The goal of my masters project is to elucidate proteins that
interact downstream of Mob1p and Dbf2p in the Mitotic Exit Network
(MEN). To date, we know that activation of the MEN results in
mitotic CDK inactivation resulting from nucleolar release of Cdc14p.
I want to clarify the events that take place between Mob1p-Dbf2p
activation and Cdc14p release. I plan on doing this by means of
affinity column chromatography via a TAP tagged Mob1p in order
to purify proteins that complex with Mob1p-Dbf2p , followed by
mass spectrometry to identify these proteins. Functional testing
will ensue, but because I don't have any idea of what types of
proteins will be identified from mass spectrometry, I don't know
what types of functional experiments I'll do. Also, Mob1p and
Dbf2p translocalize from the spindle pole body to the mother-bud
neck prior to cytokinesis, so there is a strong possibility that
I'll pull out proteins involved in cytokinesis.
